Solutions for In Situ BioRemediation

Emulsified Vegetable Oil for In Situ Bioremediation

SDC-9

Bioaugmentation Culture for Groundwater Remediation

SDC-9 is a field-proven bioaugmentation culture designed to complete the anaerobic biodegradation of chlorinated solvents. It contains multiple strains of Dehalococcoides mccartyi (Dhc), the only known organisms capable of fully dechlorinating compounds like PCE and TCE to non-toxic ethene.

For sites where Dhc are absent—or present but underperforming—SDC-9 provides a reliable way to accelerate dechlorination and reach cleanup goals more efficiently.

SDC-9 canister
Product Sheet

Why SDC-9

Complete Dechlorination

Provides the Dhc required to fully break down chlorinated solvents to ethene, not just stall at intermediate compounds like cis-DCE or vinyl chloride.

Faster Treatment Progress

Mixed microbial cultures support Dhc growth, reducing lag time and increasing dechlorination rates compared to isolated cultures.

Improved Electron Donor Efficiency

Works synergistically with electron donors, helping convert available substrate into effective dechlorination rather than wasted fermentation.

Performs in Challenging Conditions

Remains active at pH levels as low as 5.5, with optimal performance above 6.0.

Technical Details

How It Works

SDC-9 introduces a complete microbial consortium into the subsurface, including Dhc and supporting organisms that provide essential growth factors and metabolic intermediates.

This coordinated microbial system enables:

  • Efficient hydrogen utilization
  • Sustained Dhc activity
  • Complete reductive dechlorination to ethene

Even at sites with native Dhc, bioaugmentation can significantly improve performance by increasing population density and metabolic efficiency.

Contaminants Treated

Chlorinated Solvents

    • PCE, TCE
    • cis-DCE, trans-DCE, 1,1-DCE
    • Vinyl Chloride (VC)

Chlorinated Alkanes & Related Compounds

    • 1,1,1-TCA, 1,1,2,2-TeCA
    • 1,2-DCA
    • Carbon Tetrachloride (CT), Chloroform (CF), Dichloromethane (DCM)

Other Compounds

    • Freon 11, Freon 113
    • HCFCs, TFE

Application

SDC-9 is typically injected between rounds of anaerobic water and electron donor to minimize oxygen exposure and improve distribution throughout the treatment zone.

Recommended Dose:

  • 1 × 10⁷ Dhc cells per liter in target zones

      Key Microbial Components

      • Dehalococcoides mccartyi (Dhc)
      • Dehalobacter
      • Dehalobium chlorocoercia
      • Desulfovibrio spp.
      • Desulfitobacterium spp.
      • Methanogens and sulfate-reducing bacteria

      Product Characteristics

      • Cell Count: ≥ 1 × 10¹¹ Dhc cells/L
      • pH: 6.0 – 8.0
      • Density: 0.9 – 1.1 g/cm³
      • Appearance: Light greenish, murky liquid
      • Odor: Musty

        Packaging & Handling

        • Shipped in 19 L (5-gallon) stainless steel kegs
        • Available in concentrated (up to 10×) formats
        • Pressurized with nitrogen and stored chilled
        • Requires inert gas (nitrogen or argon) for delivery

        Safety

        SDC-9 is a naturally occurring, non-toxic, non-pathogenic microbial consortium with no known exposure hazards. Standard PPE is recommended during handling.

        Use SDC-9 when:

        • Native Dhc are absent or too slow
        • Dechlorination stalls at DCE or VC
        • You need faster, more reliable site closure

        Typically paired with:

        • Newman Zone electron donors
        • Newman Zone OS for oxygen removal

        Need Help Selecting the Right Product?

        Talk with our team about your site conditions, contaminants, and treatment approach to determine the right product.

        Request Technical Support